mugshot-morrison-sq-transparent

Prof Ciaran Morrison

Personal Professor
SFI Principal Investigator

Research interests

  • How DNA damage impacts on centrosome duplication, cohesion and ciliogenesis
  • Roles of the centrosomal/ pericentriolar material components, Pericentrin and Mcph1, in cell cycle control
  • Activities of centrosome proteins (centrin, Cep164, Cep290) in DNA repair
  • Regulation of centrosome and centriole cohesion

Research overview

Centrosome abnormalities and amplification are common characteristics of tumour cells. Aneuploidy and chromosomal instability are highly correlated with the appearance of multiple centrosomes.

Supernumerary centrosomes can cause mitotic abnormalities, such as the formation of multipolar spindles, potentially giving rise to abnormal chromosome segregation.

Normally, centrosome amplification is tightly regulated during the cell cycle. Previous work from our group has demonstrated that DNA damage leads to centrosome amplification. One goal of our work is to understand how DNA damage is signalled to the centrosome duplication apparatus and to define the impact this has on proliferating cells.

We want to understand whether centrosome amplification is a physiologically-relevant trigger of programmed cell death through indefinite arrest or mitotic catastrophe, or a potential contributor to genomic instability and tumourigenesis.

We also explore how specific components of the centrosome regulate DNA repair and cell cycle checkpoint activities. Several key proteins of the centriole and/or the pericentriolar material are directly involved in control of the cell cycle, before and after DNA damage and we want to establish how they carry out the different functions that they have been assigned.

We are using cell biology and reverse genetics to explore these questions.

Selected publications

  • Flach J, Bakker ST, Mohrin M, Conroy PC, Pietras EM, Reynaud D, Alvarez S, Diolaiti ME, Ugarte F, Forsberg EC, Le Beau MM, Stohr BA, Mendez J, Morrison CG, Passegue E, Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature 512: 198-202 (2014)
  • Kliszczak M, Stephan AK, Flanagan AM, Morrison CG, SUMO ligase activity of vertebrate Mms21/Nse2 is required for efficient DNA repair but not for Smc5/6 complex stability. DNA Repair 11: 799-810 (2012)
  • Dantas TJ, Wang Y, Lalor P, Dockery P, Morrison CG, Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins. J Cell Biol 193: 307-318 (2011)